Research Topics
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Research projects
Determination of fluorine in geological materials by CS-HR-AAS |
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Reduction of chemical background ionic noise in LC-MS |
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| The hyphenated technique liquid chromatography-mass spectrometry (LC-MS) is considered to be the method of choice for very broad application. However, the interference of chemical background (noise) observed in atmospheric pressure ionization (i.e. electrospray and chemical ionization) has been reported to be a problem for increasing LC-MS trace analysis. In our preliminary study, a novel technique has been developed to reduce the positive chemical noise in LC-MS, which is based on exclusive gas-phase reactions of background ions with a reactive collision gas. The exclusive reactions lead to selective reduction of chemical noise in tandem triple quadrupole LC-MS. Further investigations will focus on characterization and reduction of negative background noise, improvement of the chemical noise reduction technique, its implementation and application in trace bio-analysis. | ||
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Phospholipids analysis for microorganism characterization in biogas fermentation |
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| Profiling phospholipids has been an established method for studying the microorganism population because different bacteria produce their unique phospholipids in the cell membrane. At least it is the case for the microorganisms involved in biogas fermentation. However, the structure information of phospholipids is lost when the conventional techniques such as GC and GC-MS are applied because of the derivatization steps (hydrolysis, especially). We are going to use mainly LC-MS to quantitatively analyze phospholipids in biogas fermentation. | ||
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Cooperations
Joanneum Research; Institute of Sustainable Techniques and Systems |
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Medical University of Graz |
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Equipment
Liquid chromatography-mass spectrometry |
API2000 triple quadrupole tandem MS (Applied Biosystems / MDS Sciex, Foster City, USA) coupled with Agilent 1100 HPLC system and an autosampler (Agilent, Waldbronn, Germany) |
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Liquid chromatography |
1100 HPLC with autosampler and various detection (Agilent, Waldbronn, Germany) |
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Gas chromatography |
5890 HP (Agilent) | |
Gas chromatography-mass spectrometry |
6890 GC with 5973 mass selective detector (Agilent) |
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Headspace sampler |
HS 40 (Perkin Elmer) |
